Part:BBa_I765010:Experience
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Applications of BBa_I765010
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UNIQ3beca7d8a78727e8-partinfo-00000000-QINU UNIQ3beca7d8a78727e8-partinfo-00000001-QINU
This biobrick was sequenced by the Wageningen UR 2012 team. The sequencing result showed that this biobrick is lacking its iron inducable promotor.
Sequencing result:
>I765010 sequence from BaseClear:
ATTACTATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATC
CTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCATCTAGAGA
AAGAGGAGAAATACTAGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGG
GTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTT
CAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCC
TGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTC
GTGACCACCTTCGGCTACGGCCTGCAATGCTTCGCCCGCTACCCCGACCA
CATGAAGCTGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCC
AGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCC
GAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGG
CATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACA
ACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGC
ATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCA
GCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGC
TGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGAC
CCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGC
CGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAATAATACTAGAGCC
AGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTT
TATCTGTTGTTTGTCGGTGAACGCTCTCTACTAAAGTCACACTGGCTCAC
CTTCGGGTGGGCCTTTCTGCGTTTATATACTAATAGCGGCCGCTGCAGGC
TTTCCTCCTCACTGACTCGCTGCCCTCGGGTCTTTGGCTGCGGCGAGCCG
TATCAGCTCATTCAAAGGGGTAATACGGTTA
XbaI cutting site: TCTAGA
RBS site (BBa_B0034):
aaagaggagaaa
As can be seen, the RBS site starts right after the XbaI cutting site (1 bp between them), meaning the iron inducable promotor is lacking completely. Nor is there any other promotor present.